The DolPHIN 2 qualitative study seeks to explore factors that influence late antenatal initiation and the adherence to and retention in cART programmes in pregnancy. The study will examine the policy, implementation and acceptability aspects of the introduction of DTG as a first-line HIV treatment in Uganda and South Africa. Qualitative in-depth interviews and focus group discussions have been conducted with a range of stakeholders in HIV treatment, including pregnant women and recent mothers who are living with HIV/AIDS and their partners, health workers, HIV policy makers, and patient advocacy organisations. The data are now analysed and full results are published. Evidence from the study will be used to make recommendations that will contribute to improving services for preventing vertical transmission and DTG-based treatment in the study countries and beyond.